Antioxidant Potential of Pleurotus porrigens Extract and Application in Sunflower Oil during Accelerated Storage

The oxidative stability of refined sunflower oil supplemented with edible wild mushroom, Pleurotus porrigens crude extract, its n-butanol fraction (n-BUT, from liquid-liquid partitioning), and semi-purified sub-fraction (SF) III and SFIV, were tested under accelerated storage conditions compared with BHA and α-tocopherol, by measuring their peroxide value, p-anisidine value, thiobarbituric acid-reactive substance, and iodine value. The total phenolic content (TPC), 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging and ferric reducing/ antioxidant power (FRAP) were also evaluated. P. porrigens’ SFIII exhibited highest DPPH scavenging, while n-BUT showed highest FRAP; TPC was found highest in crude extract. Generally, BHA and a-tocopherol are more protective in stabilizing the sunflower oil. P. porrigens’ SFIII and SFIV had short-term protective effect in secondary oxidation for -year; whilst crude extract retarded secondary oxidation (TBARS value) and extend the shelf-life upon 1 -year. P. porrigens extracts did not show similar retardation of lipid oxidation in sunflower oil as compared to α-tocopherol and BHA at 200 ppm. However, the higher concentration of P. porrigens extracts that provided the protective effect in stabilizing sunflower oil can be further studied.